$250 each

Bin1 Monoclonal Antibodies

Clones 99D (LDI-301), 2F11 (LDI-302) and 12A (LDI-303) - 100 ug each

  • Description
  • References
  • 99D
  • 2F11
  • 12A
  • To Order

Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons (1), functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein (2). Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain (2). However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions (3, 4, 5, and 6); Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoforms (7). Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a tumor suppressor and inhibits malignant cell transformation (7). Studies in mouse suggest that this gene plays an important role in cardiac muscle development (8). Bin1 has also been implicated in Alzheimer's disease and cardiac disease (9, 10). Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM: 255200]; also known as autosomal recessive myotubular myopathy (11).

Monoclonal antibodies available from LDI include splice-isoform specific antibodies 99D and 12A, for generally expressed or brain-specific isoforms, respectively; and the pan-Bin1 antibody 2F11, which recognizes all Bin1 isoforms.

  1. Lichte, B., R. et al. (1992) EMBO J. 11:2521-30.

  2. Sakamuro, D. et al. (1996) Nat. Genet. 14:69-77.

  3. Butler, M. H. J. et al. (1997) Cell Biol. 137:1355-67.

  4. Ramjaun, A. R. et al. (1997) J. Biol. Chem. 272:16700-6.

  5. Tsutsui, K. et al. (1997) Biochem. Biophys. Res. Commun. 236:178-83.

  6. Wechsler-Reya, R., D. et al. (1997) J. Biol. Chem. 272:31453-8.

  7. Prendergast G.C. et al. (2009) Biochim Biophys Acta.1795:25-36.

  8. Muller A. J. et al. (2003) Mol Cell Biol. 23:4295-306.

  9. Joseph H. L. et al. (2011) Arch Neurol. 69: 320-8.

  10. Hong T et al. (2010) PloS biology 8:e1000312.

  11. Nicot A.-S. et al. (2007) Nat. Genet. 39:1134-9.

Anti-Bin1, Clone 99D (LDI-301)

Antigen Information

Recombinant fragment corresponding to amino acids 189-398 of human Bin1

Source Antibody/Purification Information

Mouse monoclonal [99D] to Bin1, isotype IgG
Protein G column purified, 1 mg/ml

Species Cross Reactivity

Mouse, rat, human

Applications

Western, ELISA, IP, IHC (acetone-fixed frozen sections), FACS analysis

Recommended Dilutions for Each Application

Western - 1:1000
Immunoprecipitation - use at an assay dependent dilution
Immunohistochemistry - use at an assay dependent dilution

Anti-Bin1, Clone 2F11 (LDI-302)

Antigen Information

GST chimeric protein that encoded human Bin1 BAR domain

Source Antibody/Purification Information

Mouse monoclonal [2F11] to Bin1, isotype IgG1
Protein G column purified, 1mg/ml

Species Cross Reactivity

Mouse, human

Applications

Western, IP, IHC (formalin-fixed paraffin-embedded sections), ELISA

Recommended Dilutions for Each Application

Western - 1:1000
Immunoprecipitation - use at an assay dependent dilution
Immunohistochemistry - use at an assay dependent dilution

Anti-Bin1, Clone 12A (LDI-303)

Antigen Information

GST-Bin1 12A exon

Source Antibody/Purification Information

Mouse monoclonal [1810] to Bin1, isotype IgG

Species Cross Reactivity

Human

Applications

Western, ELISA

Recommended Dilutions for Each Application

Western - use at an assay dependent dilution

To Place an Order

By Phone: 484-476-3474
By Fax: 484-476-8533
By Email: sales@limrdev.com

By Mail:
LIMR Development, Inc.
Sales Department
Attn: J. Wynne
100 East Lancaster Avenue
Wynnewood, PA 19096

Payment Options:
  • Purchase Order (P.O.) - for those accounts in good standing only; net 30 days
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Please have the following information ready when placing an order using a P.O., check, or cash payment:

  1. Contact Information (please include an email for order status notification)
  2. Shipping and Billing Information
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*Will be used to determine if a credit account may be opened.

Shipping Information:

Antibody shipment is via Federal Express Standard Overnight, Monday-Thursday in the contiguous United States and Monday-Wednesday in Alaska, Hawaii, and internationally. Antibodies will not be shipped on a holiday or on the day prior to a holiday.

Shipping costs can be charged directly to your Federal Express account number. If you choose to do so, please provide your account number with your shipping address.

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