OxPhos™ Assay Kit - patent pending

KLD-002 - 100 assays

  • Description
  • References
OxPhos Assay Kit
OxPhos™ is a tissue culture medium-based novel assay that measures the glutathione recycling capacity/glutathione function of cells in culture and whole blood.

Glutathione is a natural antioxidant that limits cellular damage in healthy tissues during oxidative stress. Glutathione functions to maintain thiol redox homeostasis. It is also centrally involved in repairing damage induced by cancer drugs and radiation and in the detoxification of several commonly used cancer chemotherapeutic drugs. These processes cause altered glutathione homeostasis in both normal and cancer tissues. Therefore, glutathione homeostasis by recycling of oxidized glutathione is necessary for the survival of cells. Moreover, it is apparent that the glutathione recycling capacity rather than the level of glutathione is prognostic.

Unlike other complicated assays on the market that measure only glutathione levels, OxPhos™ measures the entire glutathione recycling capacity of your cells in just 4 easy steps. OxPhos™ has multiple applications in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.

  1. Li J, Zhang D, Ward KM, Prendergast GC and Ayene IS. Hydroxyethyl disulfide as an efficient metabolic assay for cell viability in vitro. Toxicol. In Vitro, 26(4):603-12.

  2. Ayene IS, Biaglow JE, Kachur AV, Stamato TD and Koch CJ (2008). Mutation in G6PD gene leads to loss of cellular control of protein glutathionylation: Mechanism and Implication. J. Cell Biochem. 103:123-135.

  3. Ayene IS, Stamato TD, Mauldin SK, Biaglow JE, Tuttle SW, Jenkins SF and Koch CJ (2002). Mutation in the glucose-6-phosphate dehydrogenase gene leads to inactivation of Ku DNA end binding during oxidative stress. J. Biol. Chem. 277:9929-9935.

LDI Research Products

CellCountEZ®
The easier, more effective way
to quantitate cell survival and proliferation

LIMR Antibodies

RadDose™
Chemical Dosimetry
Calibration Solution

OxPhos™
Measure the entire glutathione recycling capacity of your cells.

LIMR Cardiovascular
Research Group (CRG)
  • Cardiac Safety
    Assessment Services